Bacterial Cell Lysis Buffer, 100ml

The Bacterial Cell Lysis Kit has been developed for the extraction of biologically active, soluble proteins and inclusion bodies from bacterial cells. The Bacterial Lysis Buffer is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA & RNA) released during cell lysis. The kit’s lysis eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.

Bacterial Lysis Buffer is based on organic buffering agents and utilizes a mild nonionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into the Bacterial Lysis Buffer. It has been tested for use with several widely used bacterial strains and is recommended for E. coli and gram-negative bacteria.

The Bacterial Cell Lysis Kit includes a modified lysozyme preparation that contains nucleases and results in optimal lysis and minimal contamination. This kit contains all of the reagents needed to perform protein extraction. Bacterial Lysis Buffer is also available separately for further downstream applications.